Hotspot

rpl20-rps12

LSC · 72421-73237 · 817 bp

Back to Corylopsis

Pi 0.00346

MarkerSeek score 56.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 15
pi: 0.00346
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTCGAATGAGCCCC	CGCCGAGGAACATGTACTAG	55.0	55.0	57.8	57.9	912-939 bp	1.0	80.8
rpl20-rps12_p2	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	864-891 bp	1.0	80.1
rpl20-rps12_p3	TGTTCTACGTCTCCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.6	57.9	862-889 bp	1.0	79.7
rpl20-rps12_p4	TTGTTCTACGTCTCCGAGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.5	57.9	863-890 bp	1.0	79.1
rpl20-rps12_p5	GTAAGTCTCGAATGAGCCCC	GCCGAGGAACATGTACTAGG	55.0	55.0	57.8	57.5	911-938 bp	1.0	77.7