Hotspot

rpl33-rps18

LSC · 71287-71466 · 180 bp

Back to Corylopsis

Pi 0.0151

MarkerSeek score 55.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 19
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACGGGCGTTTCCAGATATAT	TCAATTCGATCCCCCGATTG	45.0	50.0	56.5	57.7	386 bp	1.0	70.6
rpl33-rps18_p2	ACGGGCGTTTCCAGATATATT	TCAATTCGATCCCCCGATTG	42.9	50.0	57.2	57.7	386 bp	1.0	68.6
rpl33-rps18_p3	ACGGGCGTTTCCAGATATAT	ATTCGATCCCCCGATTGGAT	45.0	50.0	56.5	58.6	383 bp	1.0	67.6
rpl33-rps18_p4	ACGGGCGTTTCCAGATATAT	CAATTCGATCCCCCGATTGG	45.0	55.0	56.5	58.8	385 bp	1.0	66.6
rpl33-rps18_p5	ACGGGCGTTTCCAGATATAT	AATTCGATCCCCCGATTGGA	45.0	50.0	56.5	58.9	384 bp	1.0	65.8