Hotspot

accD-psaI

LSC · 61954-62635 · 682 bp

Back to Corylopsis

Pi 0.004

MarkerSeek score 54.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 17
pi: 0.004
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCACGCTTTCTTTCCTTTGA	GGCACCAAAACAGAGGGTAA	42.9	50.0	58.1	58.0	754-759 bp	1.0	74.0
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	GGCACCAAAACAGAGGGTAA	50.0	50.0	58.8	58.0	759-761 bp	0.928571	71.9
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	801-803 bp	0.928571	71.1
accD-psaI_p4	GCTCCACGCTTTCTTTCCTT	CCATTGCCGGAACTACTAGG	50.0	55.0	58.8	57.8	788-790 bp	0.928571	70.0
accD-psaI_p5	GCTCCACGCTTTCTTTCCT	GGCACCAAAACAGAGGGTAA	52.6	50.0	58.1	58.0	759-761 bp	0.928571	69.5