Hotspot

psaA-ycf3

LSC · 44746-45424 · 679 bp

Back to Corylopsis

Pi 0.00225

MarkerSeek score 55.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 9
pi: 0.00225
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	848-882 bp	1.0	83.6
psaA-ycf3_p2	GTTCTTGAAAAATGGCCGGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	850-884 bp	1.0	82.8
psaA-ycf3_p3	GTTCTTGAAAAATGGCCGGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	781-815 bp	1.0	82.8
psaA-ycf3_p4	TTGAAAAATGGCCGGGTTTG	ATCAAGCCGCTGAGTATTGG	45.0	50.0	57.8	58.0	844-878 bp	1.0	82.3
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	777-811 bp	0.928571	80.0