Hotspot

trnG-GCC-trnfM-CAU

LSC · 39413-39593 · 181 bp

Back to Corylopsis

Pi 0.0121

MarkerSeek score 54.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 6
pi: 0.0121
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AAAAACGCGGATATGGTCGA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	284-293 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p2	AAAACGCGGATATGGTCGAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	283-292 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p3	AAAACGCGGATATGGTCGAA	GTCACGGGTTCAAATCCTGT	45.0	50.0	58.0	58.1	277-286 bp	1.0	83.3
trnG-GCC-trnfM-CAU_p4	AAAAACGCGGATATGGTCGA	GTCACGGGTTCAAATCCTGT	45.0	50.0	58.0	58.1	278-287 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p5	AAAACGCGGATATGGTCGAA	TCACGGGTTCAAATCCTGTC	45.0	50.0	58.0	57.8	276-285 bp	1.0	82.7