Hotspot

clpP1

LSC · 73601-75835 · 2235 bp

Back to Corydalis

Pi 0.157

MarkerSeek score 80.50

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2235
variable_sites: 1273
pi: 0.157
unique_haplotype_count: 48
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TCAACAATTGGTGGCATGGA	GTATCAATCCGCAATGCAGC	45.0	50.0	58.0	58.0	2786-2833 bp	0.285714	63.0
clpP1_p2	AACAATTGGTGGCATGGACT	GTATCAATCCGCAATGCAGC	45.0	50.0	58.0	58.0	2784-2831 bp	0.285714	63.0
clpP1_p3	TCAACAATTGGTGGCATGGA	TTTTCTACGGGCCAATGCTT	45.0	45.0	58.0	58.1	2751-2977 bp	0.285714	62.7
clpP1_p4	TACAAGGGGGAAGGTAAGGG	GTATCAATCCGCAATGCAGC	55.0	50.0	58.0	58.0	2581-2619 bp	0.244898	57.0
clpP1_p5	ATTTGCCCCTGGTTCCATTT	GTATCAATCCGCAATGCAGC	45.0	50.0	58.0	58.0	2819-2857 bp	0.204082	52.7