Hotspot

rpl32-ndhF

IRa · 159981-162519 · 2539 bp

Back to Corydalis

Pi 0.194

MarkerSeek score 73.00

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: IRa
length_bp: 2539
variable_sites: 1837
pi: 0.194
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GTACGTTTCTTTGGAACCGC	TGATCCATGCATATTGATATGT	50.0	31.8	57.7	53.1	1987-2739 bp	0.346939	25.9
rpl32-ndhF_p2	GTACGTTTCTTTGGAACCGC	ATGATCCATGCATATTGATATGT	50.0	30.4	57.7	53.8	1988-2740 bp	0.346939	25.9
rpl32-ndhF_p3	GTACGTTTCTTTGGAACCGC	TGCATATTGATATGTATGTTCCA	50.0	30.4	57.7	53.7	1980-2732 bp	0.346939	25.9
rpl32-ndhF_p4	TACGTTTCTTTGGAACCGCC	TGATCCATGCATATTGATATGT	50.0	31.8	58.8	53.1	1986-2738 bp	0.346939	25.9
rpl32-ndhF_p5	TACGTTTCTTTGGAACCGCC	ATGATCCATGCATATTGATATGT	50.0	30.4	58.8	53.8	1987-2739 bp	0.346939	25.9