Hotspot

ycf1-rps15

IRa · 147378-149779 · 2402 bp

Back to Corydalis

Pi 0.311

MarkerSeek score 63.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: IRa
length_bp: 2402
variable_sites: 1570
pi: 0.311
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.29
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TCCACGCTTGAGAATTGGTT	CGTTTTCCCCGATTCAGGAT	45.0	50.0	57.7	58.0	795-2813 bp	0.285714	55.0
ycf1-rps15_p2	CCACGCTTGAGAATTGGTTG	CGTTTTCCCCGATTCAGGAT	50.0	50.0	57.7	58.0	794-2812 bp	0.285714	54.4
ycf1-rps15_p3	TCCACGCTTGAGAATTGGTT	TTCCCCGATTCAGGATCAGT	45.0	50.0	57.7	58.1	791-2809 bp	0.285714	54.3
ycf1-rps15_p4	CCACGCTTGAGAATTGGTTG	TTCCCCGATTCAGGATCAGT	50.0	50.0	57.7	58.1	790-2808 bp	0.285714	53.8
ycf1-rps15_p5	TCCACGCTTGAGAATTGGTT	ATGCATTGATGGGTGGTGAG	45.0	50.0	57.7	58.2	867-2885 bp	0.285714	53.3