Hotspot

trnE-UUC-trnT-GGU

LSC · 39772-40336 · 565 bp

Back to Corydalis

Pi 0.068

MarkerSeek score 80.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 565
variable_sites: 237
pi: 0.068
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	382-641 bp	1.0	89.1
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	361-620 bp	1.0	88.9
trnE-UUC-trnT-GGU_p3	CCTGAACCACTAGACGATGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.4	57.9	358-617 bp	1.0	86.5
trnE-UUC-trnT-GGU_p4	CTGAACCACTAGACGATGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.4	57.9	357-616 bp	1.0	86.5
trnE-UUC-trnT-GGU_p5	TGAACCACTAGACGATGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.8	57.9	356-615 bp	1.0	84.7