Hotspot

trnC-GCA-petN

LSC · 35784-36654 · 871 bp

Back to Corydalis

Pi 0.0671

MarkerSeek score 82.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 348
pi: 0.0671
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	792-975 bp	1.0	89.1
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.5	58.0	767-950 bp	1.0	88.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	786-969 bp	0.979592	86.8
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.5	57.8	761-944 bp	0.979592	85.8
trnC-GCA-petN_p5	TTCAAATCTGGGTGTCGCC	TTAAAGCAGCCCAAGCAAGA	52.6	45.0	58.1	58.0	765-948 bp	1.0	83.5