Hotspot

rpoB-trnC-GCA

LSC · 34472-35712 · 1241 bp

Back to Corydalis

Pi 0.0729

MarkerSeek score 79.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 515
pi: 0.0729
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	TTGTTGTTGATCAGGCGACA	50.0	45.0	58.0	58.0	1797-1878 bp	0.877551	86.6
rpoB-trnC-GCA_p2	CCCTTAGGGTCCAACTCTGA	AGAATCCTTCCTTGGAGCCT	55.0	50.0	58.0	58.0	1915-1976 bp	0.44898	69.4
rpoB-trnC-GCA_p3	TCCCTTAGGGTCCAACTCTG	AGAATCCTTCCTTGGAGCCT	55.0	50.0	58.0	58.0	1916-1977 bp	0.44898	69.4
rpoB-trnC-GCA_p4	CCGGGGCTTTGCAATATTTG	AGAATCCTTCCTTGGAGCCT	50.0	50.0	58.1	58.0	1882-1943 bp	0.44898	69.3
rpoB-trnC-GCA_p5	CGAGCCCATATCCTTGCTTT	AGAATCCTTCCTTGGAGCCT	50.0	50.0	58.0	58.0	2002-2063 bp	0.428571	68.8