Hotspot

trnV-UAC-rps16

LSC · 10999-12120 · 1122 bp

Back to Corydalis

Pi 0.443
MarkerSeek score 72.30
Primer available yes
Label trnV-UAC-rps16

Feature Metadata

label_name
trnV-UAC-rps16
feature_type
igs
region
LSC
length_bp
1122
variable_sites
1099
pi
0.443
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.88
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-rps16_p1 TAGGCTACAAATGGACCCCA CCACCGAGATTGTTCACGAT 50.0 50.0 58.0 58.0 640-1683 bp 0.306122 64.0
trnV-UAC-rps16_p2 AGCTTGACTTTTGGTTCGGT CCACCGAGATTGTTCACGAT 45.0 50.0 57.9 58.0 678-1722 bp 0.306122 63.7
trnV-UAC-rps16_p3 GGTTCGAATCCGTATAGCCC CCACCGAGATTGTTCACGAT 55.0 50.0 57.9 58.0 188-1231 bp 0.306122 63.6
trnV-UAC-rps16_p4 TAGGCTACAAATGGACCCCA GGGCTGTGTGAGAAACTTCA 50.0 50.0 58.0 58.0 1506-1651 bp 0.265306 55.8
trnV-UAC-rps16_p5 AGCTTGACTTTTGGTTCGGT GGGCTGTGTGAGAAACTTCA 45.0 50.0 57.9 58.0 1545-1690 bp 0.265306 55.7