Hotspot

atpH

LSC · 11840-12085 · 246 bp

Back to Corybas

Pi 0.0138

MarkerSeek score 40.10

Primer available yes

Label atpH

Feature Metadata

label_name: atpH
feature_type: gene
region: LSC
length_bp: 246
variable_sites: 7
pi: 0.0138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH_p1	TACACAACTCGCACACACTC	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.2	57.9	1053-1218 bp	1.0	89.6
atpH_p2	TCTAAAGAAAGTGCAGCCGG	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.2	57.9	1096-1261 bp	1.0	89.0
atpH_p3	TCGAGATTGTGCACCCTTTT	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.7	57.9	1149-1315 bp	1.0	88.3
atpH_p4	TTGTGCACCCTTTTCCCTAG	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.7	57.9	1143-1309 bp	1.0	88.2
atpH_p5	AGTCCTTCCCGGAGTATTGT	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	827-831 bp	0.8	71.9