Hotspot

psaC-rps15

IRa · 105038-106004 · 967 bp

Back to Corybas

Pi 0.0395

MarkerSeek score 88.50

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: IRa
length_bp: 967
variable_sites: 90
pi: 0.0395
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 88.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	AGCTCGAACACATTGGGTAC	TTCTGGGAAAACGTCAACGA	50.0	45.0	57.9	57.7	1001-1219 bp	1.0	87.0
psaC-rps15_p2	TCGAACACATTGGGTACACC	TTCTGGGAAAACGTCAACGA	50.0	45.0	57.8	57.7	998-1216 bp	1.0	86.4
psaC-rps15_p3	GCTCGAACACATTGGGTACA	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.2	57.7	1000-1218 bp	1.0	86.2
psaC-rps15_p4	AGCTCGAACACATTGGGTAC	ACCCGGGAATCAAAAACTCG	50.0	50.0	57.9	58.5	918-1136 bp	1.0	86.2
psaC-rps15_p5	AGCTCGAACACATTGGGTAC	CCCGGGAATCAAAAACTCGT	50.0	50.0	57.9	58.5	917-1135 bp	1.0	86.2