Hotspot

rpl32-psaC

IRa · 104356-104791 · 436 bp

Back to Corybas

Pi 0.161

MarkerSeek score 68.70

Primer available yes

Label rpl32-psaC

Feature Metadata

label_name: rpl32-psaC
feature_type: igs
region: IRa
length_bp: 436
variable_sites: 134
pi: 0.161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-psaC_p1	TCCTAAGAGCAGCGTGTCTA	GGCCCTTTCTCACAAACAGA	50.0	50.0	57.9	58.0	1293-1552 bp	1.0	90.9
rpl32-psaC_p2	TCCTAAGAGCAGCGTGTCTA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	975-1234 bp	1.0	90.8
rpl32-psaC_p3	AGCAGCGTGTCTACCAATTT	GGCCCTTTCTCACAAACAGA	45.0	50.0	57.8	58.0	1286-1545 bp	1.0	90.4
rpl32-psaC_p4	AATTTCACCATGGCGGCTTA	AGATGTGAATCCGCTTGTCC	45.0	50.0	58.2	58.0	953-1212 bp	0.8	82.5
rpl32-psaC_p5	AATTTCACCATGGCGGCTTA	GGCCCTTTCTCACAAACAGA	45.0	50.0	58.2	58.0	1271-1530 bp	0.8	81.2