Hotspot

clpP-psbB

LSC · 62481-62983 · 503 bp

Back to Corybas

Pi 0.0447

MarkerSeek score 83.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 51
pi: 0.0447
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	599-640 bp	1.0	88.7
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	545-586 bp	1.0	87.9
clpP-psbB_p3	ACCCAAACTGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	585-626 bp	1.0	87.2
clpP-psbB_p4	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	602-643 bp	1.0	86.8
clpP-psbB_p5	TACGTCAACCCAAACTGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	57.7	58.2	592-633 bp	1.0	86.6