Hotspot

MFP44_pgt021-psaJ

LSC · 57294-57583 · 290 bp

Back to Corybas

Pi 0.0481
MarkerSeek score 82.90
Primer available yes
Label MFP44_pgt021-psaJ

Feature Metadata

label_name
MFP44_pgt021-psaJ
feature_type
igs
region
LSC
length_bp
290
variable_sites
28
pi
0.0481
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
MFP44_pgt021-psaJ_p1 ACCCGTGACATTTTGTACCC CCTGCTAAAGACCCAAACCA 50.0 50.0 58.1 57.7 400-416 bp 1.0 86.4
MFP44_pgt021-psaJ_p2 CCCGTGACATTTTGTACCCA CCTGCTAAAGACCCAAACCA 50.0 50.0 58.1 57.7 399-415 bp 1.0 86.4
MFP44_pgt021-psaJ_p3 ACCCGTGACATTTTGTACCC ACCTGCTAAAGACCCAAACC 50.0 50.0 58.1 57.7 401-417 bp 1.0 86.3
MFP44_pgt021-psaJ_p4 CCCGTGACATTTTGTACCCA ACCTGCTAAAGACCCAAACC 50.0 50.0 58.1 57.7 400-416 bp 1.0 86.3
MFP44_pgt021-psaJ_p5 ACAGGATTTGAACCCGTGAC CCTGCTAAAGACCCAAACCA 50.0 50.0 58.1 57.7 411-427 bp 1.0 86.1