Hotspot

MFP44_pgt020-MFP44_pgt021

LSC · 57098-57219 · 122 bp

Back to Corybas

Pi 0.0557

MarkerSeek score 83.70

Primer available yes

Label MFP44_pgt020-MFP44_pgt021

Feature Metadata

label_name: MFP44_pgt020-MFP44_pgt021
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 14
pi: 0.0557
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MFP44_pgt020-MFP44_pgt021_p1	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	178-223 bp	1.0	88.1
MFP44_pgt020-MFP44_pgt021_p2	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	176-221 bp	1.0	88.1
MFP44_pgt020-MFP44_pgt021_p3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	179-224 bp	1.0	88.0
MFP44_pgt020-MFP44_pgt021_p4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	189-234 bp	1.0	87.9
MFP44_pgt020-MFP44_pgt021_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	190-235 bp	1.0	87.8