Hotspot

accD-psaI

LSC · 50545-51054 · 510 bp

Back to Corybas

Pi 0.0423

MarkerSeek score 77.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 510
variable_sites: 49
pi: 0.0423
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATACCCGACGGTTTACAAGC	AATGAACAGACCTGCGATCC	50.0	50.0	58.0	58.0	1264-1318 bp	0.8	78.1
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	AATGAACAGACCTGCGATCC	50.0	50.0	58.0	58.0	1156-1210 bp	0.8	78.0
accD-psaI_p3	GTGAGAAAATCGCCCGTTTG	AATGAACAGACCTGCGATCC	50.0	50.0	58.0	58.0	1572-1626 bp	0.8	77.4
accD-psaI_p4	CACGGTTTCCTTCCCTTGAA	TAGGCCCACTAAAGGAACGA	50.0	50.0	58.0	58.1	592-603 bp	0.8	75.5
accD-psaI_p5	ATACCCGACGGTTTACAAGC	TAGGCCCACTAAAGGAACGA	50.0	50.0	58.0	58.1	700-711 bp	0.8	75.3