Hotspot

rpoB-MFP44_pgt006

LSC · 24554-25376 · 823 bp

Back to Corybas

Pi 0.0645

MarkerSeek score 84.70

Primer available yes

Label rpoB-MFP44_pgt006

Feature Metadata

label_name: rpoB-MFP44_pgt006
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 120
pi: 0.0645
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-MFP44_pgt006_p1	CCCTCATTTTTCTTCTGGAGC	TAAAGGATTTGCAGTCCCCC	47.6	50.0	56.9	57.5	771-890 bp	1.0	70.7
rpoB-MFP44_pgt006_p2	TCCCTCATTTTTCTTCTGGAGC	TAAAGGATTTGCAGTCCCCC	45.5	50.0	58.3	57.5	772-891 bp	1.0	69.3
rpoB-MFP44_pgt006_p3	CCCTCATTTTTCTTCTGGAGCA	TAAAGGATTTGCAGTCCCCC	45.5	50.0	58.6	57.5	771-890 bp	1.0	67.2
rpoB-MFP44_pgt006_p4	CCCTCATTTTTCTTCTGGAGC	ATAAAGGATTTGCAGTCCCCC	47.6	47.6	56.9	58.0	772-891 bp	1.0	66.6
rpoB-MFP44_pgt006_p5	TCCCTCATTTTTCTTCTGGAGC	ATAAAGGATTTGCAGTCCCCC	45.5	47.6	58.3	58.0	773-892 bp	1.0	65.3