Hotspot

atpH-atpI

LSC · 12086-12478 · 393 bp

Back to Corybas

Pi 0.0656

MarkerSeek score 80.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 63
pi: 0.0656
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	AACTTTGGCTGCGGCTTATA	45.0	45.0	58.0	57.9	472-477 bp	1.0	90.7
atpH-atpI_p2	CCTCTGCTTCTGGTTGTCTC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.9	57.9	579-584 bp	1.0	89.7
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	GTCAGGCGCTCTTTTACCTT	45.0	50.0	58.0	58.2	838-843 bp	1.0	87.5
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	GGGCCTTGGGTTCCATTTAT	45.0	50.0	58.0	57.8	894-899 bp	1.0	87.3
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	ATCCACAAACCATTCCGACC	45.0	50.0	58.0	58.2	989-994 bp	1.0	86.5