Hotspot

MFP44_pgt003-MFP44_pgt004

LSC · 6995-7430 · 436 bp

Back to Corybas

Pi 0.0612

MarkerSeek score 64.40

Primer available yes

Label MFP44_pgt003-MFP44_pgt004

Feature Metadata

label_name: MFP44_pgt003-MFP44_pgt004
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 42
pi: 0.0612
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.58
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MFP44_pgt003-MFP44_pgt004_p1	CAATCCGACGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	310-565 bp	1.0	59.9
MFP44_pgt003-MFP44_pgt004_p2	AATCCGACGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	309-564 bp	1.0	59.9
MFP44_pgt003-MFP44_pgt004_p3	CAATCCGACGCTTTAGTCCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	311-566 bp	1.0	58.8
MFP44_pgt003-MFP44_pgt004_p4	AATCCGACGCTTTAGTCCAC	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	310-565 bp	1.0	58.8
MFP44_pgt003-MFP44_pgt004_p5	CGGAGAGAGAGGGATTCGAA	TACCACTAAACTATACCCGC	55.0	45.0	58.3	54.0	363-618 bp	1.0	56.8