Hotspot

matK-MFP44_pgt001

LSC · 3081-3992 · 912 bp

Back to Corybas

Pi 0.0447

MarkerSeek score 83.10

Primer available yes

Label matK-MFP44_pgt001

Feature Metadata

label_name: matK-MFP44_pgt001
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 90
pi: 0.0447
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-MFP44_pgt001_p1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	45.0	50.0	58.0	57.9	1216-1282 bp	1.0	89.7
matK-MFP44_pgt001_p2	TCGCGACGAGAATGGAATTT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.0	58.2	1199-1265 bp	1.0	89.3
matK-MFP44_pgt001_p3	TCGCGACGAGAATGGAATTT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.0	58.2	1198-1264 bp	1.0	89.3
matK-MFP44_pgt001_p4	TCGCGACGAGAATGGAATTT	ATTGACAATGGCGTCTCGAA	45.0	45.0	58.0	57.9	1462-1525 bp	0.8	80.5
matK-MFP44_pgt001_p5	TCGCGACGAGAATGGAATTT	TACGTTTCTAGGGGGCATCT	45.0	50.0	58.0	57.8	1926-1954 bp	0.8	76.5