Hotspot

psaJ-rpl33

LSC · 64487-64878 · 392 bp

Back to Coronilla

Pi 0.0104

MarkerSeek score 61.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 4
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GTGGCACCAGTGGTAAGTAC	TCACAACCGGTACATTCCAA	55.0	45.0	58.2	57.1	550-556 bp	1.0	72.4
psaJ-rpl33_p2	CTCTATGGTTCGCGGCTTTA	TCACAACCGGTACATTCCAA	50.0	45.0	57.8	57.1	531-537 bp	1.0	72.3
psaJ-rpl33_p3	TCTATGGTTCGCGGCTTTAG	TCACAACCGGTACATTCCAA	50.0	45.0	57.8	57.1	530-536 bp	1.0	72.3
psaJ-rpl33_p4	TGGCACCAGTGGTAAGTACT	TCACAACCGGTACATTCCAA	50.0	45.0	58.3	57.1	549-555 bp	1.0	71.8
psaJ-rpl33_p5	GTGGCACCAGTGGTAAGTAC	GATCACAACCGGTACATTCCA	55.0	47.6	58.2	58.0	552-558 bp	1.0	71.7