Hotspot

psaI-pafII

LSC · 57949-58191 · 243 bp

Back to Coronilla

Pi 0.0207

MarkerSeek score 57.10

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 5
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	TGGGTCTAGTATTTCCGGCA	CCGTACTTCTTCTGACTGCC	50.0	55.0	58.1	58.0	357-359 bp	1.0	80.5
psaI-pafII_p2	TGGGTCTAGTATTTCCGGCA	AAATCACGGGGTTTTCGACA	50.0	45.0	58.1	58.0	395-397 bp	1.0	80.1
psaI-pafII_p3	GTGGGTCTAGTATTTCCGGC	CCGTACTTCTTCTGACTGCC	55.0	55.0	57.8	58.0	358-360 bp	1.0	79.7
psaI-pafII_p4	GTGGGTCTAGTATTTCCGGC	AAATCACGGGGTTTTCGACA	55.0	45.0	57.8	58.0	396-398 bp	1.0	79.3
psaI-pafII_p5	TGGGTCTAGTATTTCCGGCA	CCAGAAGAAATCACGGGGTT	50.0	50.0	58.1	57.8	402-404 bp	1.0	78.7