Hotspot

psbK-trnQ-UUG

LSC · 53372-53935 · 564 bp

Back to Coronilla

Pi 0.00541

MarkerSeek score 61.20

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 3
pi: 0.00541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	CCTCGGGCAATTTATCCAAGA	GACCAAAACCCGTTGCCTTA	47.6	50.0	58.1	58.7	683-687 bp	1.0	67.4
psbK-trnQ-UUG_p2	CCTCGGGCAATTTATCCAAGA	GAATAGCGGGACCAAAACCC	47.6	55.0	58.1	58.9	692-696 bp	1.0	65.6
psbK-trnQ-UUG_p3	CCTCGGGCAATTTATCCAAGA	ATATGCTCTGGGACGGAAGG	47.6	55.0	58.1	58.9	724-728 bp	1.0	65.2
psbK-trnQ-UUG_p4	CCTCGGGCAATTTATCCAAGA	ACCAAAACCCGTTGCCTTAC	47.6	50.0	58.1	59.0	682-686 bp	1.0	65.1
psbK-trnQ-UUG_p5	CCTCGGGCAATTTATCCAAG	GACCAAAACCCGTTGCCTTA	50.0	50.0	56.5	58.7	683-687 bp	1.0	64.4