Hotspot

trnG-UCC-trnS-GCU

LSC · 51715-52500 · 786 bp

Back to Coronilla

Pi 0.0104
MarkerSeek score 54.50
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
786
variable_sites
8
pi
0.0104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCGCACTTTTACCACT CAATCCGACGCTTTAGTCCA 42.9 50.0 58.2 58.0 842-855 bp 1.0 72.2
trnG-UCC-trnS-GCU_p2 ACGAATCGCACTTTTACCACT AATCCGACGCTTTAGTCCAC 42.9 50.0 58.2 58.0 841-854 bp 1.0 72.2
trnG-UCC-trnS-GCU_p3 ACGAATCGCACTTTTACCAC CAATCCGACGCTTTAGTCCA 45.0 50.0 56.8 58.0 842-855 bp 1.0 72.2
trnG-UCC-trnS-GCU_p4 ACGAATCGCACTTTTACCAC AATCCGACGCTTTAGTCCAC 45.0 50.0 56.8 58.0 841-854 bp 1.0 72.2
trnG-UCC-trnS-GCU_p5 ACGAATCGCACTTTTACCACT ATCGACGGAAAGAGAGGGAT 42.9 50.0 58.2 57.9 899-912 bp 1.0 71.5