Hotspot

atpH-atpF

LSC · 46912-47438 · 527 bp

Back to Coronilla

Pi 0.0076

MarkerSeek score 61.80

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 4
pi: 0.0076
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GAGGCAGAGGGGAAAATACG	CAGATGGCCAGTAACCCAAG	55.0	55.0	58.0	58.2	693-701 bp	1.0	78.3
atpH-atpF_p2	GCAGAGGGGAAAATACGAGG	CAGATGGCCAGTAACCCAAG	55.0	55.0	58.0	58.2	690-698 bp	1.0	78.3
atpH-atpF_p3	GGCAGAGGGGAAAATACGAG	CAGATGGCCAGTAACCCAAG	55.0	55.0	58.0	58.2	691-699 bp	1.0	78.3
atpH-atpF_p4	GCAGAGGGGAAAATACGAGG	CCAGTAACCCAAGCAAAGGA	55.0	50.0	58.0	57.7	683-691 bp	1.0	77.9
atpH-atpF_p5	GGCAGAGGGGAAAATACGAG	CCAGTAACCCAAGCAAAGGA	55.0	50.0	58.0	57.7	684-692 bp	1.0	77.9