Hotspot

trnG-GCC-psbZ

LSC · 24341-24964 · 624 bp

Back to Coronilla

Pi 0.0065
MarkerSeek score 49.70
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
624
variable_sites
4
pi
0.0065
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTTGTTTTTCGACCGGAAG CAACCACTCAGCCATCTCTC 50.0 55.0 58.0 58.0 1769-1771 bp 1.0 80.7
trnG-GCC-psbZ_p2 CGTTGTTTTTCGACCGGAAG TGAGAAAACCCTCCCTCCAT 50.0 50.0 58.0 57.9 1502-1504 bp 1.0 80.6
trnG-GCC-psbZ_p3 GGGTAGAGCAACTTGGTAGC CAACCACTCAGCCATCTCTC 55.0 55.0 58.0 58.0 1484-1488 bp 1.0 80.5
trnG-GCC-psbZ_p4 CCGTCTCCGCAACATTTTTC CAACCACTCAGCCATCTCTC 50.0 55.0 58.0 58.0 1426-1430 bp 1.0 80.4
trnG-GCC-psbZ_p5 CTTGAGGTCACGGGTTCAAA CAACCACTCAGCCATCTCTC 50.0 55.0 58.0 58.0 1448-1452 bp 1.0 80.4