Hotspot

ndhF

SSC · 113897-116131 · 2235 bp

Back to Cornus

Pi 0.0292
MarkerSeek score 63.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2235
variable_sites
256
pi
0.0292
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACGAAGGGGACTAAACAGGA GAGGTAAGATCCGGCGAATC 50.0 55.0 58.0 57.9 2557-2589 bp 0.371429 59.2
ndhF_p2 GCAGAATACCGTCACCCATT GAGGTAAGATCCGGCGAATC 50.0 55.0 58.0 57.9 2667-2699 bp 0.371429 58.9
ndhF_p3 GAGGGAAAGTGCGGAAGAAA GAGGTAAGATCCGGCGAATC 50.0 55.0 58.1 57.9 2606-2638 bp 0.371429 58.4
ndhF_p4 CGGAAGAAACGAGGGAAAGT GAGGTAAGATCCGGCGAATC 50.0 55.0 57.8 57.9 2616-2648 bp 0.371429 58.0
ndhF_p5 AGTCTGGATACGGCAAAACC GAGGTAAGATCCGGCGAATC 50.0 55.0 57.9 57.9 2804-2817 bp 0.257143 51.0