Hotspot

rps15-ycf1

SSC · 127521-127913 · 393 bp

Back to Cornus

Pi 0.0609
MarkerSeek score 71.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
393
variable_sites
89
pi
0.0609
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACACCATGCATTGATGGGT TTGAACCCGTTCGTCTGTTT 45.0 45.0 58.1 58.0 1328-1363 bp 0.428571 66.2
rps15-ycf1_p2 CCCCGATCCATTCTTCCATG TTGAACCCGTTCGTCTGTTT 55.0 45.0 58.1 58.0 1245-1280 bp 0.428571 66.0
rps15-ycf1_p3 CTTGTAGCTGGTGCAGTCAT TTGAACCCGTTCGTCTGTTT 50.0 45.0 57.9 58.0 1273-1308 bp 0.428571 65.9
rps15-ycf1_p4 TCCCGAATACCCAACTGACT TTGAACCCGTTCGTCTGTTT 50.0 45.0 58.1 58.0 1145-1180 bp 0.371429 63.6
rps15-ycf1_p5 ACCATGCATTGATGGGTGTT TTGAACCCGTTCGTCTGTTT 45.0 45.0 58.1 58.0 1325-1360 bp 0.314286 59.7