Hotspot

rpl32-trnL-UAG

SSC · 117456-118302 · 847 bp

Back to Cornus

Pi 0.0582
MarkerSeek score 77.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
847
variable_sites
188
pi
0.0582
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCTCGAGCTTGACCAATTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1561-1654 bp 0.457143 70.0
rpl32-trnL-UAG_p2 GCCTCGAGCTTGACCAATTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1562-1655 bp 0.457143 70.0
rpl32-trnL-UAG_p3 GCCTCGAGCTTGACCAATTA GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.0 58.1 2053-2146 bp 0.457143 69.4
rpl32-trnL-UAG_p4 GCCTCGAGCTTGACCAATTA ATGCCCCGAATAAATCCACC 50.0 50.0 58.0 58.0 1904-1997 bp 0.371429 62.3
rpl32-trnL-UAG_p5 GCCTCGAGCTTGACCAATTA CACCACAGGAAAACGACTCA 50.0 50.0 58.0 58.1 2259-2352 bp 0.257143 52.0