Hotspot

ndhF-rpl32

SSC · 116132-117293 · 1162 bp

Back to Cornus

Pi 0.0608
MarkerSeek score 77.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1162
variable_sites
263
pi
0.0608
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG GAATCCCCACCCTGGAAATG 50.0 55.0 58.0 58.2 1650-1721 bp 0.457143 68.3
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA GAATCCCCACCCTGGAAATG 45.0 55.0 58.0 58.2 1648-1719 bp 0.457143 68.2
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA GAATCCCCACCCTGGAAATG 45.0 55.0 58.0 58.2 1649-1720 bp 0.457143 68.2
ndhF-rpl32_p4 ACAAACGCCTTTTGACAAGC GAATCCCCACCCTGGAAATG 45.0 55.0 58.1 58.2 2091-2162 bp 0.457143 67.9
ndhF-rpl32_p5 CGCGATTAGTTACAAACGCC GAATCCCCACCCTGGAAATG 50.0 55.0 57.9 58.2 2102-2160 bp 0.285714 58.8