Hotspot

petA-psbJ

LSC · 66210-67198 · 989 bp

Back to Cornus

Pi 0.0571
MarkerSeek score 78.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
989
variable_sites
217
pi
0.0571
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGGATTTGGTCAGGGAGATG AACTGGCCGTTTTGATCCTT 55.0 45.0 58.0 58.0 1578-1783 bp 1.0 91.6
petA-psbJ_p2 CGGATTTGGTCAGGGAGATG ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.0 58.0 1577-1782 bp 1.0 91.6
petA-psbJ_p3 CTTGTGATAGGCCCTGTTCC ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.0 58.0 1942-2147 bp 1.0 91.6
petA-psbJ_p4 TCAGAGCTATCGCCCCAATA AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 2120-2171 bp 0.485714 66.7
petA-psbJ_p5 TCAGAGCTATCGCCCCAATA ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 2119-2170 bp 0.485714 66.7