Hotspot

trnL-UAA-trnF-GAA

LSC · 50355-50697 · 343 bp

Back to Cornus

Pi 0.0452
MarkerSeek score 74.60
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
343
variable_sites
63
pi
0.0452
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 325-422 bp 1.0 88.2
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 361-458 bp 1.0 87.8
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 58.1 376-473 bp 1.0 87.5
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 337-434 bp 1.0 87.1
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC CATGTGCCAGGAACCAGATT 50.0 50.0 57.5 58.2 379-476 bp 1.0 87.0