Hotspot

petN-psbM

LSC · 29943-31033 · 1091 bp

Back to Cornus

Pi 0.0483
MarkerSeek score 76.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1091
variable_sites
209
pi
0.0483
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TTTCACAGAGACAATGGGGC AGATCAAACAAGGATCCCGC 50.0 50.0 58.1 58.0 1947-2297 bp 0.457143 67.3
petN-psbM_p2 TTTCACAGAGACAATGGGGC TGGGAAGGTGGTCAGATGAT 50.0 50.0 58.1 58.0 1670-2017 bp 0.428571 65.9
petN-psbM_p3 GGGCCAGAATCCCTTTTTGA AGATCAAACAAGGATCCCGC 50.0 50.0 58.1 58.0 1670-2017 bp 0.4 63.5
petN-psbM_p4 GGGCCAGAATCCCTTTTTGA TGGGAAGGTGGTCAGATGAT 50.0 50.0 58.1 58.0 1393-1736 bp 0.371429 62.0
petN-psbM_p5 TCCCTTTTTGACTCTTCGCC TGGGAAGGTGGTCAGATGAT 50.0 50.0 58.1 58.0 1384-1727 bp 0.285714 57.1