Hotspot

trnS-GCU-trnR-UCU

LSC · 9176-10842 · 1667 bp

Back to Cornus

Pi 0.0356
MarkerSeek score 78.10
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1667
variable_sites
229
pi
0.0356
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1417-1750 bp 1.0 75.6
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 1416-1749 bp 1.0 75.5
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1423-1756 bp 1.0 74.9
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 1422-1755 bp 1.0 74.9
trnS-GCU-trnR-UCU_p5 ACGGAAAGAGAGGGATTCGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.2 56.9 1471-1804 bp 1.0 74.4