Hotspot

rps16-trnQ-UUG

LSC · 6207-7859 · 1653 bp

Back to Cornus

Pi 0.0531
MarkerSeek score 80.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1653
variable_sites
346
pi
0.0531
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1318-1835 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1322-1839 bp 1.0 85.5
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1310-1827 bp 1.0 85.1
rps16-trnQ-UUG_p4 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1317-1834 bp 1.0 84.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1571-1865 bp 0.914286 82.0