Hotspot

trnK-UUU-rps16

LSC · 4294-5066 · 773 bp

Back to Cornus

Pi 0.0504
MarkerSeek score 80.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
773
variable_sites
150
pi
0.0504
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG TGTTGCTGCCGTTTTGAAAG 50.0 45.0 58.0 58.1 1507-1559 bp 1.0 91.0
trnK-UUU-rps16_p2 CTCATGGGAGAAGCCTTTCC TGTTGCTGCCGTTTTGAAAG 55.0 45.0 58.0 58.1 1846-1902 bp 1.0 91.0
trnK-UUU-rps16_p3 TCCCTCGCTTCATCCAAATG TCTATCCCAATGAGCCGTCT 50.0 50.0 58.0 57.9 1039-1089 bp 0.971429 89.8
trnK-UUU-rps16_p4 TCCCTCGCTTCATCCAAATG AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 896-946 bp 0.914286 88.0
trnK-UUU-rps16_p5 CTCATGGGAGAAGCCTTTCC AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1233-1289 bp 0.914286 88.0