Hotspot

psaC-ndhE

SSC · 125180-125805 · 626 bp

Back to Cornukaempferia

Pi 0.0132

MarkerSeek score 49.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 626
variable_sites: 5
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTCCCACGGTATCATTTCC	AACGCGTATCAACCAATCGA	55.0	45.0	58.4	58.0	515-760 bp	1.0	81.2
psaC-ndhE_p2	CGTCCCACGGTATCATTTCC	ACGCGTATCAACCAATCGAA	55.0	45.0	58.4	58.0	514-759 bp	1.0	81.2
psaC-ndhE_p3	CGTCCCACGGTATCATTTCC	CAACGCGTATCAACCAATCG	55.0	50.0	58.4	57.9	516-761 bp	1.0	80.7
psaC-ndhE_p4	GGCAAGCTCGTACACATTGA	AACGCGTATCAACCAATCGA	50.0	45.0	58.6	58.0	482-727 bp	1.0	80.2
psaC-ndhE_p5	GGCAAGCTCGTACACATTGA	ACGCGTATCAACCAATCGAA	50.0	45.0	58.6	58.0	481-726 bp	1.0	80.2