Hotspot

trnM-CAU-ycf2

IRb · 90906-91034 · 129 bp

Back to Cornukaempferia

Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label trnM-CAU-ycf2

Feature Metadata

label_name: trnM-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 129
variable_sites: 3
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CTCAATTCGACGATCCAGGA	50.0	50.0	58.0	57.1	202 bp	1.0	74.1
trnM-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	TCTCAATTCGACGATCCAGG	50.0	50.0	58.0	57.1	203 bp	1.0	74.1
trnM-CAU-ycf2_p3	AGTTGGGCGCTTTAACCATT	CTCAATTCGACGATCCAGGA	45.0	50.0	58.4	57.1	203 bp	1.0	71.2
trnM-CAU-ycf2_p4	AGTTGGGCGCTTTAACCATT	TCTCAATTCGACGATCCAGG	45.0	50.0	58.4	57.1	204 bp	1.0	71.2
trnM-CAU-ycf2_p5	TTGGGCGCTTTAACCATTCA	CTCAATTCGACGATCCAGGA	45.0	50.0	58.4	57.1	201 bp	1.0	71.2