Hotspot

psbT-psbN

LSC · 78239-78302 · 64 bp

Back to Cornukaempferia

Pi 0.156
MarkerSeek score 73.00
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
64
variable_sites
10
pi
0.156
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTGCTCTGGTATCTTTCGCC ATCCATTCGAAGAACACGGG 50.0 50.0 58.0 58.0 370-378 bp 1.0 83.7
psbT-psbN_p2 TATGGTGGCGAACTCAATGG ATCCATTCGAAGAACACGGG 50.0 50.0 58.0 58.0 701-709 bp 1.0 82.1
psbT-psbN_p3 ATTGTGAGAGCCGATGTTCC ATCCATTCGAAGAACACGGG 50.0 50.0 58.0 58.0 779-787 bp 1.0 81.9
psbT-psbN_p4 GGAATTGTGAGAGCCGATGT ATCCATTCGAAGAACACGGG 50.0 50.0 58.0 58.0 782-790 bp 1.0 81.9
psbT-psbN_p5 TTGCTCTGGTATCTTTCGCC TTAGTGGTGTAGTCCCCCAG 50.0 55.0 58.0 58.1 723-731 bp 1.0 81.5