Hotspot

psbB-psbT

LSC · 77944-78130 · 187 bp

Back to Cornukaempferia

Pi 0

MarkerSeek score 43.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	ATTGTGAGAGCCGATGTTCC	AGAACACGGGGACTAGTTGA	50.0	50.0	58.0	58.0	769-777 bp	1.0	82.1
psbB-psbT_p2	GGAATTGTGAGAGCCGATGT	AGAACACGGGGACTAGTTGA	50.0	50.0	58.0	58.0	772-780 bp	1.0	82.1
psbB-psbT_p3	GGGGATGGAATAGCTGTTGG	AGAACACGGGGACTAGTTGA	55.0	50.0	58.0	58.0	898-906 bp	1.0	81.9
psbB-psbT_p4	GGGGATGGAATAGCTGTTGG	ATCCATTCGAAGAACACGGG	55.0	50.0	58.0	58.0	908-916 bp	1.0	81.7
psbB-psbT_p5	CAGGGTCAATGGATAACGGG	AGAACACGGGGACTAGTTGA	55.0	50.0	58.0	58.0	915-923 bp	1.0	81.7