Hotspot

clpP1-psbB

LSC · 75944-76416 · 473 bp

Back to Cornukaempferia

Pi 0.409
MarkerSeek score 91.70
Primer available yes
Label clpP1-psbB

Feature Metadata

label_name
clpP1-psbB
feature_type
igs
region
LSC
length_bp
473
variable_sites
192
pi
0.409
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
91.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1-psbB_p1 ACCCAAGTTGCATCTTCCTC GCAACTAGGGCTGTATGCAT 50.0 50.0 57.8 58.0 627-1169 bp 1.0 86.4
clpP1-psbB_p2 ATCTTCCTCTCCGGGATTCC GCAACTAGGGCTGTATGCAT 55.0 50.0 58.3 58.0 616-1158 bp 1.0 85.8
clpP1-psbB_p3 AAGTTGCATCTTCCTCTCCG GCAACTAGGGCTGTATGCAT 50.0 50.0 57.6 58.0 623-1165 bp 1.0 84.9
clpP1-psbB_p4 ACCCAAGTTGCATCTTCCTC CGATACCAAGGCAAACCCAT 50.0 50.0 57.8 58.2 555-1097 bp 1.0 84.7
clpP1-psbB_p5 ATCTTCCTCTCCGGGATTCC CGATACCAAGGCAAACCCAT 55.0 50.0 58.3 58.2 544-1086 bp 1.0 84.2