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psbJ-psbL

LSC · 67697-67817 · 121 bp

Back to Cornukaempferia

Pi 0.0331

MarkerSeek score 71.50

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 4
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	AGAGATGAACCCAACCCAGA	GCTATGACAGAATCAAATCCGA	50.0	40.9	58.0	56.3	360 bp	1.0	51.1
psbJ-psbL_p2	GTACCCACCAACCAAAGAGG	GCTATGACAGAATCAAATCCGA	55.0	40.9	58.1	56.3	285 bp	1.0	51.0
psbJ-psbL_p3	ACCAGTTACAGTACCCACCA	GCTATGACAGAATCAAATCCGA	50.0	40.9	57.9	56.3	295 bp	1.0	50.8
psbJ-psbL_p4	TACCCACCAACCAAAGAGGA	GCTATGACAGAATCAAATCCGA	50.0	40.9	58.2	56.3	284 bp	1.0	50.3
psbJ-psbL_p5	AGAGATGAACCCAACCCAGA	TGTTGAATTGAATCGTACCAGTC	50.0	39.1	58.0	57.1	328 bp	1.0	49.8