Hotspot

ndhC-trnV-UAC

LSC · 53537-55178 · 1642 bp

Back to Cornukaempferia

Pi 0.0065
MarkerSeek score 66.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1642
variable_sites
10
pi
0.0065
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTAACCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1691-1780 bp 1.0 82.6
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1690-1779 bp 1.0 82.6
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1684-1773 bp 1.0 81.8
ndhC-trnV-UAC_p4 CCTAACCGGGGCTAAAACTC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1773-1862 bp 1.0 80.4
ndhC-trnV-UAC_p5 CTAACCGGGGCTAAAACTCC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1772-1861 bp 1.0 80.4