Hotspot

psbC-trnS-UGA

LSC · 37957-38104 · 148 bp

Back to Cornukaempferia

Pi 0.0473
MarkerSeek score 79.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
148
variable_sites
7
pi
0.0473
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CTTTCCATGACCCCTCTTCG GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.0 58.0 263 bp 1.0 81.7
psbC-trnS-UGA_p2 CATTTGTGGCATGCAGGAAG GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.0 58.0 341 bp 1.0 81.4
psbC-trnS-UGA_p3 CATTTGTGGCATGCAGGAAG GCTGAGTGGTTGATAGCTCC 50.0 55.0 58.0 58.1 333 bp 1.0 81.2
psbC-trnS-UGA_p4 CATTTGTGGCATGCAGGAAG GGCTGAGTGGTTGATAGCTC 50.0 55.0 58.0 58.1 334 bp 1.0 81.2
psbC-trnS-UGA_p5 CATTTGTGGCATGCAGGAAG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.0 58.1 326 bp 1.0 81.2