Hotspot

psbM-trnD-GUC

LSC · 31540-32589 · 1050 bp

Back to Cornukaempferia

Pi 0.0173

MarkerSeek score 69.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1050
variable_sites: 18
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1178-1183 bp	1.0	63.6
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1182-1187 bp	1.0	62.9
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	45.0	55.0	55.8	58.4	1184-1189 bp	1.0	60.7
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1185-1190 bp	1.0	60.4
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1161-1166 bp	1.0	60.3