Hotspot

rps16-trnQ-UUG

LSC · 5875-7297 · 1423 bp

Back to Cornukaempferia

Pi 0.00662

MarkerSeek score 66.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1423
variable_sites: 9
pi: 0.00662
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1470-1514 bp	1.0	83.0
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1468-1512 bp	1.0	83.0
rps16-trnQ-UUG_p3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1476-1520 bp	1.0	82.9
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1452-1496 bp	1.0	82.2
rps16-trnQ-UUG_p5	CAGCCGATCATGTCCTTCAA	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.0	57.8	1474-1518 bp	1.0	81.9