Hotspot

ycf1

SSC · 121671-127238 · 5568 bp

Back to Corispermum

Pi 0.00386

MarkerSeek score 55.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 41
pi: 0.00386
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATCGTCAGACCATTCGGG	CCCCAAAAGGCGAAACAAAA	50.0	45.0	58.0	57.7	208 bp	1.0	78.8
ycf1_p2	AAATCGTCAGACCATTCGGG	AAAAACCCCCAAAAGGCGAA	50.0	45.0	58.0	58.8	214 bp	1.0	74.9
ycf1_p3	AAATCGTCAGACCATTCGGG	AAAACCCCCAAAAGGCGAAA	50.0	45.0	58.0	58.8	213 bp	1.0	74.9
ycf1_p4	AAATCGTCAGACCATTCGGG	AAAACCCCCAAAAGGCGAA	50.0	47.4	58.0	58.1	213 bp	1.0	72.7
ycf1_p5	AAATCGTCAGACCATTCGGG	AAACCCCCAAAAGGCGAAA	50.0	47.4	58.0	58.1	212 bp	1.0	72.7